琼脂糖凝胶微球在多酚类天然产物靶点钓取与分离中的应用
Journal: Agricultural Science DOI: 10.32629/as.v9i6.4104
Abstract
多酚类天然产物具有多种生物活性,但因天然产物组分复杂、作用靶点不明确,制约 了功能化开发与临床转化。琼脂糖凝胶微球凭借优良的生物相容性、低非特异性吸 附及丰富的可修饰羟基,已成为多酚类天然产物靶点鉴定与分离纯化的核心载体。本 文系统综述了琼脂糖凝胶微球在多酚类天然产物研究中的应用进展,重点论述基于环 氧活化与CNBr(溴化氰)活化的亲和钓靶应用,涵盖姜黄素、杨梅素、山奈酚、甘草查 尔酮、木犀草素、棉皮素等典型多酚化合物的直接作用靶点验证案例；同时介绍了 免疫亲和层析在大豆苷元、柚皮苷、黄芩苷分离纯化中的应用,以及离子交换色谱在 多酚代谢酶分离中的辅助作用。本文旨在为多酚类天然产物的分子机制解析与功能 化研究提供方法学参考。
Keywords
琼脂糖凝胶微球；多酚类天然产物；亲和钓靶；免疫亲和层析；靶点蛋白
Funding
(2024X004-KXY)北京科技职业大学院内科技类一般课题- 基于复合蛋白增强红树莓花色苷稳定性研究。
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[5] 王怡婷,刘芬芬,赵雯靓.多酚微胶囊化技术及其体外释放研究进展[J].药物化学,2 022,10(4):299-306.
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[7] Gericke,M.;Witzler,M.;Enkelmann,A.;Heinze,T.Functional Agarose Hydrogels Obtained by Employing Homogeneous Synthesis Strategies[J].Polymers 2024,16(13),1812.
[8] Fu,L.;Xiao,Q.;Ru,Y.;Hong,Q.;Weng,H.;Zhang,Y.;Chen,J.;Xiao,A. Bio-based active packaging:Gallic acid modified agarose coatings in grass carp(Ctenopharyngodon idellus)preservation[J].International Journal of Biological Macromolecules 2024,255,128196.
[9] Alács,B.;Zrinyi,A.;Hornyánszky,G.;Poppe,L.;Bell,E.Upgrading Epoxy Supports for Enzyme Immobilization by Affinity Function Doping—A Case Study with Phenylalanine Ammonia-Lyase from Petroselinum crispum[J].Catalysts 2024,14(1),14.
[10] Firouzi,Z.;Lari,P.;Rashedinia,M.;Ramezani,M.;Iranshahi,M.;Abnous, K. Proteomics screening of molecular targets of curcumin in mouse brain[J].Life sciences2014,98(1),12-17.
[11] Wang,Z.;Hsieh,T.-c.;Zhang,Z.;Ma,Y.;Wu,J.M.Identification and purification of resveratrol targeting proteins using immobilized resveratrol affinity chromatography[J].Biochemical and biophysical research communications 2004,323 (3),743-749.
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[13] 刘倩,寇俊萍,黄娅琳.以人参皂苷Rg1为配基的亲和色谱介质的制备与初步应用[J ].中国药科大学学报2010,41(5),451-455.
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[15] Rodriguez,E.L.;Poddar,S.;Iftekhar,S.;Suh,K.;Woolfork,A. G.;Ovbude,S.;Pekarek,A.;Walters,M.;Lott,S.;Hage,D.S. Affinity chromatography:A review of trends and developments over the past 50 years[J].Journal of Chromatography B 2020,1157,122332.
[16] Hou,D.-X.;Kumamoto,T.Flavonoids as protein kinase inhibitors for cancer chemoprevention: direct binding and molecular modeling[J].Antioxidants & redox signaling 2010,13(5),691-719.
[17] Lee,C.-J.;Moon,S.-J.;Jeong,J.-H.;Lee,S.;Lee,M.-H.;Yoo, S.- M.;Lee,H.S.;Kang,H.C.;Lee,J.Y.;Lee,W.S. Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis[J].Cell Death & Disease2018,9(3),401.
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[19] Hayakawa,R.;Ishii,T.;Fushimi,T.;Kamei,Y.;Yamaguchi,A.; Sugimoto,K.;Ashida,H.;Akagawa,M.Luteolin protects human ARPE-19 retinal pigment epithelium cells from blue light-induced phototoxicity through activation of Nrf2/Keap1 signaling[J].Free Radical Research 2025,59(4),356- 368.
[20] Wang,L.;Wang,X.;Chen,H.;Zu,X.;Ma,F.;Liu, K.;Bode,A.M.;Dong,Z.;Kim,D.J.Gossypin inhibits gastric cancer growth by direct targeting of AURKA and RSK2[J].Phytotherapy Research2019,33(3),640- 650.
[21] Qu,H.;Qu,B.;Wang,X.;Zhang,Y.;Cheng,J.;Zeng,W.;Liu,S.;Wang,Q.;Zhao,Y.Ra pid, sensitive separation of the three main isoflavones in soybean using immunoaffinity chromatography [J].Journal of Separation Science 2016,39(6),1195-1201.
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[23] Zhao,Y.;Feng,H.;Shan,W.;Cheng,J.;Wang,X.;Zhao,Y.;Qu,H.;Wang,Q.Developm ent of immunoaffinity chromatography to specifically knockout baicalin from Gegenqinlian Decoction [J].Journal of Separation Science 2015,38(15),2746- 2752.
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[26] Unterlander,N.;Mats,L.;McGary,L.C.;Gordon,H.O.W.;Bozzo,G.G.Kaempferol rhamnoside catabolism in rosette leaves of senescing Arabidopsis and postharvest stored radish [J]. Planta2022,256(2):36.
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