江苏某奶牛场奶牛乳房炎病原细菌的分离鉴定及药敏特性分析
Journal: Agricultural Science DOI: 10.32629/as.v9i3.3836
Abstract
为明确江苏某奶牛场奶牛乳房炎的致病病原菌种类及其药物敏感性,为临床合理用药提供科学依据,本研究采集该奶牛场患病奶牛乳汁样本,通过细菌分离纯化、菌落形态观察、革兰氏染色镜检、PCR扩增鉴定及药敏试验等方法进行系统研究。结果显示,从奶样中分离获得一株优势菌株,该菌株在TSA琼脂培养基上形成直径0.5~1.0 mm、圆形凸起、表面光滑、边缘整齐、呈金黄色或灰白色的不透明菌落；革兰氏染色呈紫色,为革兰氏阳性菌,菌体呈小球形,以葡萄串状、短链状排列或单个存在；PCR扩增获得1492 bp的16S rRNA基因片段,经NCBI数据库BLAST比对,该菌株与葡萄球菌同源性达100%,鉴定为葡萄球菌。药敏试验结果表明,该分离菌株对头孢拉定、头孢氨苄、氟苯尼考、青霉素、头孢噻肟表现为高度敏感；对万古霉素、甲氧氨苄表现为中度敏感；对林可霉素表现为耐药。本研究明确了该奶牛场本次奶牛乳房炎的致病菌为葡萄球菌,且筛选出了有效敏感的抗菌药物,为该场奶牛乳房炎的临床精准用药提供了依据。
Keywords
奶牛乳房炎；葡萄球菌；分离鉴定；药敏试验；耐药性
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