棘胸蛙谷氧还蛋白基因家族的克隆及表达分析
Journal: Agricultural Science DOI: 10.12238/as.v8i1.2670
Abstract
本研究鉴定和分析了棘胸蛙(Quasipaa spinosa)谷氧还蛋白基因家族(QsGrx1、QsGrx2、QsGrx3和QsGrx5),并研究该基因家族在棘胸蛙物种中的表达规律。通过实时荧光定量分析Grx基因家族在各组织中的表达量情况,研究棘胸蛙Grx基因家族在两种病原菌作用下的表达调控规律。结果发现Grx基因家族均含有Glutaredoxin结构域,QsGrx3还存在Thioredoxin结构域,除QsGrx5外均含有糖基化位点及配体,通过三维结构建模的发现蛋白质结构均由五个α螺旋和四个β片组成。实时荧光定量分析表明各基因(QsGrx1、QsGrx2、QsGrx3和QsGrx5)分别在肾脏,脾脏和胃,肺和精巢,精巢中表达量最高,肌肉中表达量最低。QsGrx1和QsGrx3对弗氏柠檬酸杆菌反应较小,QsGrx2和QsGrx5分别在弗氏柠檬酸杆菌注射后72h和12h、72h mRNA相对表达量最高；在布氏柠檬酸杆菌注射前期QsGrx1和QsGrx3反应较明显,而后期QsGrx2和QsGrx5反应较明显。结果表明棘胸蛙的Grx基因家族在氧化还原稳态以及宿主先天免疫防御系统中起着至关重要的作用。
Keywords
棘胸蛙；谷氧还蛋白；微生物胁迫；基因表达分析
Funding
福建省自然科学基金项目(2020J01370)；福建省高校“福建省高等学校新世纪优秀人才支持计划”(闽教科(2018)47号)。
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[7] Wingert RA, Galloway JL, Barut B, et al. Deficiency of glutaredoxin 5 reveals Fe–S clusters are required for verte brate haem synthesis[J].Nature 2005,436(7053):1035-1039.
[8] Bandeira Junior G, dos Santos AC, Souza CdF, et al. Citrobacter freundii infection in silver catfish (Rhamdia quelen): Hematological and histological alterations[J]. Micro bial Pathogenesis2018,125:276-280.
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[12] Martin JL. Thioredoxin —a fold for all reasons[J]. Structure1995,3(3):245-250.
[13] Fu Y,Xiang Y, Li H, et al. Inflammation in kidney repair: Mechanism and therapeutic potential[J].Pharmacology & Thera peutics 2022, 237:108240.
[14] Li R, Zeng J, Ren T. Expression of DEL-1 in alveolar epithelial cells prevents lipopolysaccharide-induced inflamm ation, oxidative stress, and eosinophil recruitment in acute lung injury[J]. International Immunopharmacology 2022, 110: 108961.
[15] Doreswamy K, Muralidhara. Genotoxic consequences associated with oxidative damage in testis of mice subjected to iron intoxication[J].Toxicology 2005,206(1):169-178.
[16] Fan R, Li Y, Jiang S, et al. cDNA cloning and expression analysis of glutaredoxin 3 in black tiger shrimp Penaeus monodon[J].Aquaculture International 2021,29(6):2661-2679.
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