Comparative Study on In Vitro Toxicity of E-Cigarette Liquids to CHO and Respiratory Cells
Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v6i3.4434
Abstract
With the increasing popularity of electronic cigarettes, the potential health risks of e-cigarette liquids have attracted widespread attention. This study aimed to compare the in vitro toxicity of e-cigarette liquids from different brands to Chinese Hamster Ovary (CHO) cells, upper respiratory tract cells (RPMI2650), and lower respiratory tract cells (HPF) through a series of cytotoxicity assays. Six detection methods (MTT, LDH, BrdU incorporation, WST-1, CCK8, and neutral red phagocytosis assay) were used to evaluate cell viability, membrane integrity, proliferation, and metabolic activity.The results are expected to provide experimental basis for understanding the toxic effects of e-cigarette liquids on different cell types and assessing their potential risks to human health.
Keywords
in vitro toxicity, e-cigarette liquids, CHO, respiratory cells
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[3] Anand R,B. A S,Kenneth W, et al. Modeled Respiratory Tract Deposition of Aerosolized Oil Diluents Used in Δ9-THC-Based Electronic Cigarette Liquid Products [J]. Frontiers in Public Health, 2021, 9 744166-744166.
[4] William D Bennett, Phillip W Clapp, et al. Acute Effect of E-Cigarette Inhalation on Mucociliary Clearance in E-Cigarette Users [J]. Aerosol Med Pulm Drug Deliv.2024,37(4):167-170.
[5] Constantine V,Manolis T,Alexander V, et al. Evaluation of respiratory irritants among the most popular e-cigarette refill liquids across 9 European countries [J]. EUROPEAN RESPIRATORY JOURNAL, 2017, 50 (s61):OA1978.
[6] W P C,A E P,T J L, et al. Flavored e-cigarette liquids and cinnamaldehyde impair respiratory innate immune cell function. [J]. American journal of physiology. Lung cellular and molecular physiology, 2017, 313 (2): 278-292.
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