荧光探针技术在分析化学教学中的应用案例研究--以共轭聚噻吩为例
Journal: Journal of International Education Forum DOI: 10.12238/jief.v7i2.12818
Abstract
共轭聚噻吩(PT)凭借其独特的π-共轭结构、可调控的生物相容性及环境刺激响应特性,成为极具潜力的荧光探针材料,用于分析物检测。其通过静电、疏水等作用与目标分析物结合后,可引发骨架构象或聚集状态变化,紫外-可见分光光度法可检测光学响应。本研究以聚噻吩为案例载体,系统探讨了荧光探针技术在分析化学教学中的创新应用模式。研究重点分析了共轭聚噻吩探针的检测机制及其在分子检测中的典型案例,将其融入创新项目和课程的教学,有助于学生的创新思维培养。
Keywords
生物分析教学；共轭聚噻吩；光学生物传感技术；教学案例
Funding
华北理工大学大学生创新创业项目(X2024206)。
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[2] Yamamoto T, Sanechika K, Yamamoto A. Preparation of thermostable and electric-conducting poly(2,5-thienylene) [J].J.Polym.Sci.,Part C:Polym.Lett.,1980,18(1):9-12.
[3] Shu X,Li Z X,Xia J B.Synthetic Methods for Poly(thiophe ne)s[J].Progress in Chemistry,2015,27(4):385-394.
[4] Elsenbaumer R L,Jen K Y,Oboodi R.Processible and env ironmentally stable conducting polymers[J]. Synth. Met., 1986, 15(2-3):169-174.
[5] Ho H A,Dore K,Boissinot M,et al.Direct molecular detect ion of nucleic acids by fluorescence signal amplification [J]. J.Am.Chem.Soc.,2005,127(36):12673-12676.
[6] Kell A J,Page L, Tan S, et al. The development of a silica nanoparticle-based label-free DNA biosensor [J]. Nanoscale, 2011,3(9):3747-3754.
[7] Ho H A, Boissinot M, Bergeron M G, et al. Colorimetric and fluorometric detection of nucleic acids using cationic polythiophene derivatives[J].Angew. Chem.,Int.Ed.,2002,41(9):1548-1551.
[8] Ho H A,Leclerc M.Optical sensors based on hybrid apta mer/conjugated polymer complexes[J].Journal of the American Chemical Society,2004,126(5):1384-1387.
[9] Abérem M B, Najari A, Ho H A, et al. Protein detecting arrays based on cationic polythiophene-DNA-aptamer comple xes[J].Advanced Materials,2006,18(20):2703.
[10] Yao Z Y,Bai H, Li C, et al. Colorimetric and fluorescent dual probe based on a polythiophene derivative for the dete ction of cysteine and homocysteine[J].Chemical Communicati ons,2011,47(26):7431-7433.
[11] Tang Y L,Teng F,Yu M H, et al. Direct visualization of glucose phosphorylation with a cationic polythiophene [J]. Advanced Materials,2008,20(4):703.
[12] Yao Z Y,Feng X L, Hong W J, et al. A simple approach for the discrimination of nucleotides based on a water-solu ble polythiophene derivative[J].Chem.Commun.,2009,(31):4696-4698.
[13] Huang B H,Geng Z R,Ma X Y,et al. Lysosomal ATP imaging in living cells by a water-soluble cationic polythiophene deri vative[J].Biosensors & Bioelectronics,2016,83:213-220.
[14] Geng Z R,Zhang M M,Huang B H,et al.A novel nanopart icle fluorescent probe based on a water-soluble conjugated polymer for real-time monitoring of ATP fluctuation and configuration of the Golgi apparatus during the inhibition of glycolysis[J].Analytica Chimica Acta,2024,1304.
[15] Liu C, Zhang Q, An N Q, et al. A new water-soluble polythiophene derivative as a probe for real-time monitoring adenosine 5′-triphosphatase activity in lysosome of living cells[J].Talanta,2018,182:396-404.
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