早期糖尿病SD大鼠玻璃体腔注射康柏西普对血液VEGF及炎症因子影响
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v8i1.18556
Abstract
目的：探讨糖尿病SD大鼠模型玻璃体腔注射康柏西普(KH902)后,全身血管内皮生长因子(VEGF)及炎症因子白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)的短期动态变化及安全性。评估不同病程阶段对上述指标的影响。方法：选取6周龄SD大鼠,随机分为正常组与糖尿病(DM)组。将建模成功的糖尿病SD大鼠和正常组大鼠根据病程及给药后取样时间分为6个亚组(6周正常/DM组24h、96h,8周正常/DM组96h),每组11只。随后将各亚组随机分为KH902组(n=6)与PBS组(n=5)。采用多因子检测试剂盒测定血清中VEGF、IL-6、IL-10、TNF-α的平均荧光强度(MFI)。使用SPSS 27.0软件进行统计分析,P<0.05为差异有统计学意义。结果：DM组中,给药24h时,给药组血清IL-10水平高于PBS组(P<0.05)。KH902组不同给药时间比较,正常组表现为IL-6在24h时高于96h(P<0.05)；DM组表现为IL-6和IL-10水平在24h时高于96h(P<0.05)。结论：玻璃体腔注射KH902可在短期内调节糖尿病SD大鼠模型的血清IL-10和IL-6水平,而未引起VEGF、TNF-α显著变化,提示其局部用药可能引起全身炎症调节效应,且短期安全性良好。此外,KH902的全身效应在本研究所涉及的不同病程阶段间未见显著差异,为糖尿病视网膜病变(DR)临床治疗的给药方案提供实验依据。
Keywords
糖尿病；糖尿病视网膜病变；血液；VEGF；炎症因子
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