SLC16A2基因缺失对U2OS骨肉瘤细胞的EMT过程的调节作用
Journal: Basic Medical Theory Research DOI: 10.12238/bmtr.v8i1.18548
Abstract
目的：本研究旨在探讨SLC16A2基因在U2OS骨肉瘤细胞上皮-间充质转化(EMT)过程中的作用。方法：通过转染技术敲低U2OS细胞中SLC16A2基因,构建SLC16A2缺失的U2OS细胞模型,进一步通过实时荧光定量PCR分析EMT相关标志物mRNA的表达。结果：qPCR结果显示,SLC16A2在U2OS细胞中敲低成功；与对照组相比,EMT相关标志物mRNA表达发生显著变化,提示SLC16A2参与调控U2OS细胞EMT过程。结论：SLC16A2基因通过调控EMT通路中的关键分子,进而降低U2OS骨肉瘤细胞的转移能力。
Keywords
SLC16A2基因；骨肉瘤；上皮-间充质转化(EMT)
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