Migration Inhibition Induced by Gypenosides and Its Combination Effect with 5-fluorouracil on Human Colon Cancer SW-620 Cells

Journal: Journal of Clinical Medicine Research DOI: 10.32629/jcmr.v2i4.547

Xiaoxia Cheng, Shao'an Xue, Zijian Wang, Fengqin He, Bo Wang

Key Laboratory of Natural Product Development and Anticancer Innovative Drug Research in Qinling, Xi'an Genetic Engineering Laboratory, College of Biological and Environmental Engineering, Xi′an University, Xi′an 710065, Shaanxi, China

Abstract

In this study we investigate the migration inhibition of Gypenosides (Gyp) and its combined effects with 5-fluorouracil (5-FU) on human colon cancer SW-620 cells, hoping to explore more potential clinical use of Gyp. Our data implied Gyp could significantly inhibit the migration potential of SW-620 cells including down-regulating matrix Metalloproteinases expression and decreasing cells adhesion ability. What’s more, evidence showed cells treated with Gyp exerted serious microfilament network collapse as well as a significant decline in the number of microvilli. A significant migration inhibitory effect was seen in Gyp groups along with the decline of cell adhesion. Further, the combination studies suggested Gyp could synergistically enhance the antitumor effect of 5-FU in SW-620 cells through the apoptosis way. The present study indicated Gyp could prevent cell migration and further enhance the cell killing effect of 5-FU on human colon cancer SW-620 cells.

Keywords

Gypenosides, SW-620 cells, anti-migration, 5-FU, synergisticeffect

Funding

National Natural Science Foundation of China; Natural Science Basic Research Project of Shaanxi; Shaanxi Department of Education "Special Project for Emergent Public Health Safety"

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Copyright © 2021 Xiaoxia Cheng, Shao'an Xue, Zijian Wang, Fengqin He, Bo Wang

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